Assay to measure the latent reservoir of replication-competent HIV-1 in suppressed patients based on ultra deep sequencing
نویسندگان
چکیده
Background: Viral outgrowth assay (VOA) is a widely used culture assay to measure the latent HIV-1 reservoir harboring replication-competent HIV-1 in resting CD4+ T cells in patients on HAART. However, the assay is costly, and both labor and resource intensive. To overcome some of these issues with the VOA, we designed an assay using ultra deep sequencing (UDS), which directly analyzes the number of different sequences of the induced viruses to score the number of latently HIV-infected resting CD4+ T cells. In this study, we tested the premise whether the viral sequences derived from two different proviruses are genetically distinct, since the assay involves a bulk culture.
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